Ching-Wei Chang, Ph.D.Research Scientist -
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OBJECTIVE
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I am a passionate Research Scientist currently seeking new career opportunities in a variety of areas:
· Mathematical modeling of complex biological systems · Enhancement of fluorescence detection in tissues and cells · Optical diagnosis of disease · Digital image processing and image informatics · Disease treatments and drug developments using molecular biology techniques |
EDUCATION
PROFESSIONAL EXPERIENCE |
University of Michigan Ann Arbor, Michigan
2005-2009 Ph.D. in Biomedical Engineering Dissertation: Improving Accuracy and Precision in Biological Applications of Fluorescence Lifetime Imaging Microscopy University of Michigan Ann Arbor, Michigan 2003-2004 M.S. in Biomedical Engineering Thesis: A Novel Technique to Identify Gene Regulation Profile by Using Nascent RNA Quantification and Microarray Analysis National Taiwan University Taipei, Taiwan 1997-2001 B.S. in Chemical Engineering Thesis: Analysis on the Permeation Characteristics of Chitosan Films Postdoctoral Scholar - Cell Imaging, Microscopy, Image Analysis, and Molecular Biology Scientist 2010-present Department of Bioengineering, University of California, Berkeley Use of FRET probes to detect protein activation and pico-scale force events after live-cell mechanical stimulation and redistribution; study of isoform-specific contributions of molecular motors to cytoskeletal mechanics; co-management of a confocal / multi-photon laser scanning microscopy system, with Dr. Sanjay Kumar Research Associate - Data Analysis, Image Denoising, and Cancer Cell Biology Scientist 2010 Department of Biomedical Engineering, University of Michigan Technical consulting and training provided to graduate students on FLIM instrumentation, FLIM data analysis, image denoising, and cell culturing, with Dr. Mary-Ann Mycek Research Fellow - FLIM, FRET, and Image Processing Algorithms Scientist 2009-2010 Department of Biomedical Engineering, University of Michigan Advanced studies based on my Ph.D. work on the improvements of the accuracy and precision of FLIM and FRET; broader applications of these techniques to other biomedical imaging systems such as CT, PET, and confocal / multi-photon microscopy, with Dr. Mary-Ann Mycek. Guest Lecturer - Biomedical Optics 2009 Biomed Eng 552, University of Michigan Overview and principles of light microscopy; theory on resolution in light microscopy Teaching Assistant - Analytical & Quantitative Light Microscopy 2007, 2009 Marine Biological Laboratory Courses / laboratories on FLIM and FRET, designed for scientists, researchers, and advanced graduate students Research Assistant - Bioinformatics, Genomics, and Transcriptomics 2005 Department of Radiation Oncology, University of Michigan Novel gene expression profiling technique and regulation of glucose transporters by p53, with Dr. Mats Ljungman Research Assistant - Computational / Systems Biology and Mathematical Modeling 2002-2003 Institute of Chemistry, Academia Sinica, Taipei, Taiwan Theoretical analysis and computer simulation for genetic regulatory networks, with Dr. Chao-Ping Hsu |
LABORATORY TECHNIQUES
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Imaging / Microscopy
Confocal and multi-photon microscopy, time-gated and TCSPC FLIM, FRET, atomic force microscopy (AFM), super resolution microscopy (PALM/STORM), traction force microscopy (TFM), fluorescence recovery after photobleaching (FRAP), ultrasound, CT, MRI Image Processing Image segmentation and morphometric analysis, TV and wavelet-based image denoising, image deconvolution, optimal gating and fitting methods for fluorescence lifetime mapping Cellular / Molecular Biology Cell culture, DNA microarray techniques / data analysis, total / nascent RNA isolation / amplification, DNA recombination, real-time PCR, immunocytofluorescence, western blot, site-directed mutagenesis, viral transduction, transient transfection, RNAi, flow cytometry, FACS Tissue Engineering / Biomaterials Collagen-based biomaterial manufacturing as articular cartilage phantoms, chitosan-based biomaterial manufacturing and crosslinking, freeze drying, SEM, microbe passage test, bubble point test, water uptake test, mechanical strength test Chemical Separation Membrane separation, HPLC, GC, TLC |
COMPUTER SKILLS
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Languages / Programming
Matlab, LabView, C, C++, Java, Fortran Operation Systems Windows 95/98/2000/XP/Vista/7/8, Macintosh, Linux, Unix Language programming was extensively applied to image processing and the computational sections of my Ph.D. dissertation, mainly with Matlab. The control of electronics was implemented with LabView. C and Java were used to simulate complex molecular interactions described by chemical kinetics. |
LANGUAGES
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Fluent in English and Chinese (Mandarin); Fair in Taiwanese; Beginner-level in Japanese
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